技术平台系列培训2025年第六期：生物大分子相互作用系统Biacore T200培训通知

生物大分子相互作用系统Biacore T200培训通知

Biacore基于表面等离子共振（SPR）技术，具有实时检测、无需标记、卓越的灵敏度、可靠的数据质量等特点，能够检测的分子类型十分丰富，包括小分子化合物、蛋白、核酸、多糖等几乎所有的生物分子。

可应用于1、动力学分析：精确测定生物分子相互作用的结合速率常数（ka）、解离速率常数（kd），从而计算出亲和力常数（KD），为深入了解分子间作用机制提供关键数据。

2、亲和力测定：可准确评估不同生物分子对之间亲和力的强弱，帮助筛选高亲和力的分子对，在药物靶点发现与优化中具有重要意义。

3、浓度测定：通过与已知浓度的参考样品对比，能够对未知样品中的生物分子浓度进行定量分析。

4、特异性分析：能有效区分特异性结合与非特异性结合，确定生物分子相互作用的特异性，为研究复杂生物体系提供有力支持。

培训时间和地点：2025.3.20（周四），A302

报名方式：请以课题组为单位集中报名至lqye@siii.cas.cn。由于场地限制，将根据报名情况分组培训。

报名截止时间：2024.3.19，17:00

联系人：叶琳芊，54923034

公共技术中心

公共仪器与测试分析平台

Training Notice for Biomolecular Interaction Analysis System Biacore T200

Biacore is based on the surface plasmon resonance (SPR) technology. It features real-time detection, label-free operation, excellent sensitivity, and reliable data quality. It can detect a wide variety of molecular types, including almost all biomolecules such as small molecule compounds, proteins, nucleic acids, polysaccharides, etc.

It can be applied in the following aspects:

1. Kinetic analysis: Precisely determine the association rate constant (ka) and dissociation rate constant (kd) of biomolecular interactions, and thus calculate the affinity constant (KD), providing key data for a deep understanding of the molecular interaction mechanism.

2. Affinity determination: Accurately evaluate the strength of affinity between different biomolecular pairs, helping to screen high-affinity molecular pairs, which is of great significance in drug target discovery and optimization.

3. Concentration determination: By comparing with reference samples of known concentrations, the concentration of biomolecules in unknown samples can be quantitatively analyzed.

4. Specificity analysis: It can effectively distinguish specific binding from non-specific binding, determine the specificity of biomolecular interactions, and provide strong support for the study of complex biological systems.

Training time and location: March 20, 2025 (Thursday), A302

Registration method: Please register as a unit of the research group to lqye@siii.cas.cn. Due to venue limitations, training will be grouped based on the number of applicants.

Registration deadline: 17:00, March 20, 2025.

Contact: YE Linqian, 021-54923034

Institutional Center for Shared Technologies and Facilities

Analytical Core Facility