公共技术服务平台系列讲座2021年第三期：宽场反卷积技术在活细胞成像中的应用

宽场反卷积技术在活细胞成像中的应用 

　　作为世界上最早的高端高分辨活细胞成像系统——DeltaVision系统采用领先的还原型反卷积成像技术，将宽场显微镜的分辨率和灵敏度均提升到新的高度，在获得与激光共聚焦同等高分辨率的情况下，将光毒性和光淬灭维持在最低状态，因此尤其适合进行高分辨率的长时间活细胞成像，以及固定样品的荧光成像，特别是能够对小的、暗的和活的样品进行高分辨率成像。高分辨率活细胞成像系统主要应用于细胞生物学、微生物学和病毒学等研究领域。其对细胞内囊泡等微小结构和微弱信号的优秀探测能力极大地拓展了科研工作的广度和深度。 　　  

讲座内容： 

1. 介绍宽场还原型反卷积技术的应用 

2. 介绍宽场还原型反卷积技术的原理和样品制备 　　  

主讲人： 童昕--徕卡显微系统应用工程师 

时间：2021年6月18日13:30-15:00 

地点：A201报告厅 

　　公共技术服务平台

　　公共仪器与测试分析中心

　　Application of wide field deconvolution technique in living cell imaging　　  

As the world's first high-end high-resolution imaging system for living cells, DeltaVision system adopts the leading reduced deconvolution imaging technology, which improves the resolution and sensitivity of the wide field microscope to a new level, and keeps the phototoxicity and light quenching to a minimum while obtaining the same high resolution as laser confocal imaging, Therefore, it is especially suitable for high-resolution imaging of living cells for a long time and fluorescence imaging of fixed samples, especially for small, dark and living samples. High resolution living cell imaging system is mainly used in cell biology, microbiology and virology. Its excellent ability to detect intracellular vesicles and other micro structures and weak signals has greatly expanded the breadth and depth of scientific research. 

Lecture content: 

1. Introduce the application of wide field reduction deconvolution technology. 

2. Explain the principle of wide field reduction deconvolution and sample preparation. 

Lecturer: Xin Tong, Application engineer of Leica Microsystems 

Time: 13:30-15:00, June 18, 2021 

Venue: A201 lecture hall 

Public Technical Platform Office

Instrumental Analysis Center