试运行通知：毛细管电泳分析系统 PA800 Plus

试运行通知：毛细管电泳分析系统 PA800 Plus

研究所新购置的毛细管电泳分析系统 PA800 Plus已完成安装调试，将于2024年8月19日至9月19日进行为期一个月的试运行阶段。在此期间，免收机时费用但须在SAMP网站预约使用。毛细管电泳分析系统配备有UV、PDA、LIF检测器，具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点。可用于：

1. 不同片段mRNA完整性分析、环状RNA纯度分析、mRNA加帽效率、尾巴分布分析等；

2. 蛋白的纯度分析，蛋白等电点电荷异质性分析；

3.质粒超螺旋纯度分析，用于检测质粒的不同构象；

4. 腺相关病毒AAV衣壳蛋白的纯度分析；

5. 非生物药领域的应用包括：如阴阳离子分析，化药辅料环糊精取代度，PTA，乳制品等的研究。

毛细管电泳分析系统属于大型仪器，在未经培训考核的情况下，不允许个人自主上机。请有试用需求的用户联系仪器管理员叶琳芊 (A314室)。如您有任何疑问或需求，欢迎随时与我们咨询联系。

技基础支撑中心

核酸药物递送平台

Notice for Trial Operation: Capillary Electrophoresis Analyzer PA800 Plus

The newly purchased Capillary Electrophoresis Analyzer PA800 Plus has been installed, and is planning a one-month trial operation phase from August 19 to September 19, 2024. During this period, the microscope will be free of use but users need to make reservation in SAMP website. Capillary Electrophoresis Analyzer PA800 Plus is equipped with UV, PDA, and LIF detectors, and it has the advantages of higher efficiency, faster speed, less sample and reagent consumption, and equally broad application scope. It can be used for the following applications:

1. Analysis of the integrity and purity of different fragment mRNA, circular RNA, mRNA capping efficiency, and tail distribution;

2. Purity analysis of proteins, charge heterogeneity analysis of protein isoelectric point;

3. Purity analysis of plasmid supercoils to detect different conformations of plasmids;

4. Purity analysis of AAV capsid protein;

5. Non-biological drug applications, including ion analysis, drug excipient cyclodextrin substitution degree, PTA, dairy products, etc.

Capillary Electrophoresis Analyzer PA800 Plus is a large-scale instrument, and individuals are not allowed to operate it independently without training and assessment. Please contact the instrument administrator Ye Linqian (A314) for users with trial needs. If you have any questions or needs, feel free to contact us.

Technology Foundation Support Center

Nucleic Acid Drug Delivery Facility