技术平台系列培训2025年第十期：激光共聚焦显微镜成像原理及应用培训通知

激光共聚焦显微镜成像原理及应用培训通知

研究所内一台转盘共聚焦显微镜 （Olympus SpinSR10）以及一台高内涵细胞成像分析系统（Olympus FV-1200），将安排EVIDENT技术应用专家于2025年4月23日入所做培训。

Olympus SpinSR10 采用新型的Nipkow系统，可实现多色长时间超高分辨（120 nm）成像，成像速度快（200 fps），光毒性极低，适合长时间活细胞成像。本套设备配置了两个量子效率峰值高达95%的背照式sCMOS相机，配备了405 nm, 488 nm, 561 nm, 640 nm四根全固体长寿命激光器。搭载了硬件超分辨模块，搭配专利的软件超高分辨算法及100× NA 1.5的万能平场复消色差高分辨油浸物镜，共同实现转盘超高功能。

Olympus FV-1200配置405 nm、488 nm、559 nm、635 nm激光器，可进行固定或活组织和细胞的微分干涉及荧光的断层扫描，多重荧光的断层扫描及重叠，实时动态扫描及其动态构建，荧光光谱分析，荧光各项指标定量分析，组织与细胞的三维结构动态构建，荧光共振能量转移(FRET)的分析，光漂白后的荧光修复(FRAP)等。

使用这两套设备的实验人员必须依次参加理论培训、上机培训、带教，并通过考核。设备采取预约、双签登记的方式。

报名范围及培训要求

1. 理论培训不限范围，可到场签到，集中培训。

2. 上机操作培训，近3个月内需要使用该仪器开展实验的人员。

3. 申请上机操作培训者，必须已参加理论培训。上机培训限12人，每个课题组一个名额。同组内先报先得。

培训安排

主讲人：EVIDENT 徐昊工程师

理论培训：2025/4/23 9:00-11:00，地点：B505

上机培训：2025/4/23 13:30-14:30 FV-1200，地点：A324

上机培训：2025/4/23 14:30-15:30 SpinSR10，地点：A326

答疑时间：2025/4/23 15:30-16:30 地点：A326

报名方式：扫描二维码填写报名表，报名截止日期：4月22日17:00。

联系人：赵璟（A314）  电话：54923033 邮箱: jzhao1@siii.cas.cn。

公共技术中心

公共仪器与测试分析平台

Training Notice for Principle and Application of Laser Confocal Microscope Systems

The Analytical Core Facility is scheduling a training session for the Olympus SpinSR10 and Olympus FV-1200 on April 23, 2025 with the EVIDENT Technical Applications Specialist.

Olympus SpinSR10 adopts a new Nipkow double turntable system, which can realize multi-color long-time ultra-high resolution (120 nm) imaging, fast imaging speed (200 fps), extremely low phototoxicity, suitable for long-term living cell imaging. It is equipped with two back illuminated CMOS cameras with peak quantum efficiency up to 95%, and four all solid-state long-life lasers of 405 nm, 488 nm, 561 nm and 640 nm are equipped. It is equipped with hardware super-resolution module, patented software ultra-high-resolution algorithm and 100×NA 1.5 universal flat field achromatic high-resolution oil immersed objective lens to achieve the turntable ultra-high function.

Olympus FV-1200 is equipped with 405 nm, 488 nm, 559 nm, 635 nm lasers. It can perform differential interference and fluorescence tomography of fixed or living tissues and cells, multi fluorescence tomography and overlapping, real-time dynamic scanning and dynamic construction, fluorescence spectrum analysis, quantitative analysis of various fluorescence indexes, dynamic construction of three-dimensional structure of tissue and cell, and fluorescence resonance energy transfer (FRET) analysis, fluorescence repair after photobleaching (FRAP) and so on.

The experimental personnel must participate in theoretical course, hands-on training, and pass the assessment before obtaining authorization. The equipment is available by appointment and double-signature registration.

Application scope

1. Theoretical course is not limited, and you can sign up offline.

2. Hands-on training, personnel who need to use the instrument to carry out experiments in the next 3 months.

3. If you need hands-on training, the theoretical course is necessary. Only 12 persons can take part in the hands-on training. One for each group.

Training arrangements

Speaker: XU Hao, EVIDENT technical application.

1. Theoretical course in Chinese: 2025/04/23 9:00-11:00 a.m.

2. Hands-on Sessions in Chinese:

2025/04/23 13:30-14:30 FV-1200 Rm. A324.

2025/04/23 14:30-15:30 SpinSR10 Rm. A326.

3. Q&A in Chinese: 2025/04/23 15:30-16:30 Rm. A326.

Ways of registration:

Scan the QR code to fill in the registration form. Deadline for registration: 5 pm. 22nd April, 2025.

Contact: ZHAO Jing (A314) Tel: 54923033, Email: jzhao1@siii.cas.cn.

Institutional Center for Shared Technologies and Facilities

Analytical Core Facility