技术平台系列培训2024年第七期：圆二色光谱仪培训通知

圆二色光谱仪培训通知

圆二色光谱是表征具有手性结构的分子的空间构象的一个基本方法，研究所的圆二色光谱仪设备（Chirascan V100）配备了停流Stopped Flow装置。欢迎大家报名参加培训。该设备广泛用于：

1. 研究生物大分子（蛋白质、DNA、RNA等）的二级结构和三级结构，研究生物大分子、有机高分子的热稳定性，研究各类手性化合物（有机物、无机物、聚合物高分子等）的手性立体结构。

2. 分子的空间结构与功能、分子间相互作用、大分子的热稳定性Tm、生物大分子的高级结构相似性比对。分子间发生反应的毫秒级的快速反应动力学机理研究等。

CD测定常用buffer推荐: 优先选用：1.磷酸缓冲液（钾盐优于钠盐）；2.醋酸缓冲液；3.碳酸氢盐Hepes（10 mM-25 mM）。

尽量避免：高浓度钠盐、有光学活性物质的缓冲体系。

培训日程：2024.5.29（星期三）

10:00-11:30，A201，理论培训

13:00-17:00，A302， 上机操作

课程讲师: 应用光物理工程师，沈超

请以课题组为单位集中报名至jshao@siii.cas.cn。由于场地限制，将根据报名情况分组上机培训。

报名截止时间：2024.5.28，17:00

联系人：邵将，54923036

科技基础支撑中心

公共仪器与测试分析平台

Training Notice for Circular Dichroism Spectrometer

Circular dichroic spectroscopy is a fundamental method for characterizing the spatial conformation of molecules with chiral structures, and the Chirascan V100 of the research institute is equipped with a stopped flow device. Welcome to register for the training. It can be used for: 

1. It can be used to study the secondary and tertiary structures of biological macromolecules, study the thermal stability of biological macromolecules and organic polymers, and study the chiral stereostructure of various chiral compounds.

2. Comparison of spatial structure and function of molecules, intermolecular interactions, thermal stability Tm of macromolecules, and high-level structural similarity of biomolecules. Research on the millisecond level rapid reaction kinetics mechanism of intermolecular reactions.

Recommended commonly used buffer for CD determination: Priority should be given to: 1. Phosphate buffer (potassium salt is better than sodium salt); 2. Acetic acid buffer solution; 3. Hepes bicarbonate (10 mM-25 mM).

Try to avoid: high concentrations of sodium salts; Buffer solution with optically active substances.

Training time and location:

2024.5.29（Wednesday）

10:00-11:30, A201, Theoretical Training

13:00-17:00, A302, Operation training

Speaker: Shen Chao, Applied Photophysics Ltd.

Registration method: Please register as an unit of the research group to jshao@siii.cas.cn. Due to venue limitations, training will be grouped based on the number of applicants.

Registration deadline: 17:00, May 28, 2024

Contact: Jiang Shao, 54923036

Technology Foundation Support Center

Analytical Core Facility