技术平台系列讲座2024年第八期：病理技术讲座

病理技术讲座

本次技术讲座由Leica厂家工程师进行病理切片、免疫组化培训讲解，帮助解决技术问题，交流技术经验，互通有无。欢迎各位老师同学前来参加！有实验问题的老师同学可以现场交流。

讲座主要内容：

一、常规制片：

1. 石蜡切片过程中的样本制备流程；

2. 冰冻切片过程中的样本制备流程；

3. 脱水机，切片机，包埋机的使用讲解以及设置答疑。

二、免疫组化染色：

1. 免疫组化/多色荧光免疫组化实验的应用；

2. 免疫组化染色仪的使用讲解以及设置答疑；

3. 多色免疫荧光实验的难点答疑。

三、设备现场教学。

主讲人：徐欣，林仕卿，Leica 工程师

时间：2024年11月13日 周三 13:30-16:30

地点：B207

联系人：邵将

公共技术中心

公共仪器与测试分析平台

Pathology Technology Lecture

This technical lecture will be conducted by Leica engineers, providing training and explanations on pathological sectioning and immunohistochemistry, helping to solve technical issues, exchange technical experiences, and share knowledge. All teachers and students are welcome to attend! Teachers and students with experimental questions can have on-site discussions.

Main lecture content:

I. Conventional preparation:

1. Sample preparation process during paraffin sectioning;

2. Sample preparation process during frozen sectioning;

3. Usage explanations and setting Q&A for dehydrators, microtomes, and embedding machines.

II. Immunohistochemistry staining:

1. Applications of immunohistochemistry / multicolor fluorescent immunohistochemistry experiments;

2. Usage explanations and setting Q&A for immunohistochemistry staining instruments;

3. Q&A on the difficulties of multicolor immunofluorescence experiments.

III. On-site equipment teaching.

Speaker: XU Xin, LIN Shiqing, Leica Technician

Date: Wednesday, November 13, 2024, 13:30-16:30

Address: B207

Contact: SHAO Jiang

Institutional Center for Shared Technologies and Facilities

Analytical Core Facility